Difference between revisions of "Part:BBa K1362002"

Latest revision as of 12:09, 26 October 2014

Sortase A circularization construct (with His6)

This part can be used to circularize any linear protein. The mRFP selection marker BBa_J04450 must be replaced by the protein of interest. After expression, the protein has to be purified and treated with TEV protease and Sortase A. Please visit our wiki for detailed [http://2014.igem.org/Team:Heidelberg/Toolbox/Circularization_Constructs information] and [http://2014.igem.org/Team:Heidelberg/Toolbox_Guide instructions].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 855
Illegal AgeI site found at 967
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1144
Illegal BsaI.rc site found at 70

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1362002 short</partinfo>
-TODO
+This part can be used to circularize any linear protein. The mRFP selection marker <partinfo>BBa_J04450</partinfo> must be replaced by the protein of interest. After expression, the protein has to be purified and treated with TEV protease and Sortase A. Please visit our wiki for detailed [http://2014.igem.org/Team:Heidelberg/Toolbox/Circularization_Constructs information] and [http://2014.igem.org/Team:Heidelberg/Toolbox_Guide instructions].
 <!-- Add more about the biology of this part here