Difference between revisions of "Part:BBa K1362002:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1362002 short</partinfo>
 
<partinfo>BBa_K1362002 short</partinfo>
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===Design Notes===
 
===Design Notes===
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TODO
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The mRFP selection marker <partinfo>BBa_J04450</partinfo>, which can be removed by restriction with BsaI, is flanked by TEV protease cleavage site (left) and a sorting signal (right) with a His6 tag. By Golden gate assembly, the selection marker can be replaced by the protein to be circularized.
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===Source===
 
===Source===
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See sources of subparts.
  
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TODO
 
  
 
===References===
 
===References===

Latest revision as of 11:58, 26 October 2014

Sortase A circularization construct (with His6)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 855
    Illegal AgeI site found at 967
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1144
    Illegal BsaI.rc site found at 70


Design Notes

The mRFP selection marker BBa_J04450, which can be removed by restriction with BsaI, is flanked by TEV protease cleavage site (left) and a sorting signal (right) with a His6 tag. By Golden gate assembly, the selection marker can be replaced by the protein to be circularized.


Source

See sources of subparts.


References