Difference between revisions of "Part:BBa K1321106:Design"
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===Source=== | ===Source=== | ||
| − | + | Phytochelatin and CBDcipA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone. | |
| + | |||
| + | The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part. | ||
===References=== | ===References=== | ||
Latest revision as of 20:16, 24 October 2014
Linker-CBDcipA-linker + Phytochelatin (PC) EC20 with T7 promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 206
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 206
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 206
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 206
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 206
Illegal NgoMIV site found at 53 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The fusion protein was cloned together using the NgoMIV and AgeI sites.
The promotor was cloned onto the protein via the XbaI/SpeI sites.
Source
Phytochelatin and CBDcipA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.
The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part.