Difference between revisions of "Part:BBa K1321106:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | The fusion protein was cloned together using the NgoMIV and AgeI sites. | |
− | + | ||
+ | The promotor was cloned onto the protein via the XbaI/SpeI sites. | ||
===Source=== | ===Source=== |
Revision as of 20:15, 24 October 2014
Linker-CBDcipA-linker + Phytochelatin (PC) EC20 with T7 promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 206
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 206
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 206
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 206
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 206
Illegal NgoMIV site found at 53 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The fusion protein was cloned together using the NgoMIV and AgeI sites.
The promotor was cloned onto the protein via the XbaI/SpeI sites.
Source
CBD10+PhytoC