Difference between revisions of "Part:BBa K1321351:Design"
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===Source=== | ===Source=== | ||
| − | Our NiBP came from BBa_K1151001. | + | Our NiBP came from BBa_K1151001. CBDcenA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone. |
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===References=== | ===References=== | ||
Latest revision as of 19:55, 24 October 2014
CBDcenA with linker fused to NiBP in Freiburg format (RFC 25)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The fusion protein was cloned together using the NgoMIV and AgeI sites.
Source
Our NiBP came from BBa_K1151001. CBDcenA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.