Difference between revisions of "Part:BBa K1371000"
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<partinfo>BBa_K1371000 short</partinfo>
 
<partinfo>BBa_K1371000 short</partinfo>
  
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The domains we standardized are the loading domain,  
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The domains we standardized are the loading domain, domains of DEBS1 and the thioesterase (TE) domain from DEBS3 (Fig. 1). The loading domain is required for the direct loading of propionyl-CoA to form a starter unit for triketide lactone. DEBS1 is a large multifunctional polypeptide composed of two modules that incorporate (2S)-methylmalonyl-CoA to form extender units. The TE domain catalyzes the intramolecular cyclization of the triketide to form the target triketide lactone. <sup>[1]</sup>The target product is expected to be detected and identified from the culture broth.<br/><br/>
  
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domains of DEBS1 and the thioesterase (TE) domain from DEBS3 (Fig. 1).
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https://static.igem.org/mediawiki/2014/e/eb/B1%EF%BC%88%E5%AF%B9%EF%BC%89.png
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The loading domain is required for the direct loading of propionyl-CoA
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to form a starter unit for triketide lactone. DEBS1 is a large
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multifunctional polypeptide composed of two modules that incorporate
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(2S)-methylmalonyl-CoA to form extender units. The TE domain catalyzes
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the intramolecular cyclization of the triketide to form the target
+
−
 
+
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triketide lactone. <sup>[1]</sup>The target product is expected to be detected
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and identified from the culture broth.<br/><br/>
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+
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https://static.igem.org/mediawiki/2014/e/eb/B1%EF%BC%88%E5%AF
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%B9%EF%BC%89.png
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:32, 24 October 2014

DEBS1

The domains we standardized are the loading domain, domains of DEBS1 and the thioesterase (TE) domain from DEBS3 (Fig. 1). The loading domain is required for the direct loading of propionyl-CoA to form a starter unit for triketide lactone. DEBS1 is a large multifunctional polypeptide composed of two modules that incorporate (2S)-methylmalonyl-CoA to form extender units. The TE domain catalyzes the intramolecular cyclization of the triketide to form the target triketide lactone. [1]The target product is expected to be detected and identified from the culture broth.

B1%EF%BC%88%E5%AF%B9%EF%BC%89.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1878
    Illegal BamHI site found at 6423
    Illegal BamHI site found at 9273
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 558
    Illegal NgoMIV site found at 2041
    Illegal NgoMIV site found at 2413
    Illegal NgoMIV site found at 2446
    Illegal NgoMIV site found at 2919
    Illegal NgoMIV site found at 3628
    Illegal NgoMIV site found at 4501
    Illegal AgeI site found at 369
    Illegal AgeI site found at 1731
    Illegal AgeI site found at 2077
    Illegal AgeI site found at 2656
    Illegal AgeI site found at 4377
    Illegal AgeI site found at 4447
    Illegal AgeI site found at 5074
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1483
    Illegal BsaI.rc site found at 3913
    Illegal SapI.rc site found at 2748