Difference between revisions of "Part:BBa K1362107"

Latest revision as of 16:22, 24 October 2014

RBS + NpuDnaE C-intein RFC[105] assembly construct with additional N-teminal insertion site

This assembly construct is part of the iGEM team Heidelberg 2014's intein toolbox. It allows for easy generation of translational units of the Npu DnaE C-intein flanked by N and C terminal POIs as described in RFC[105]. This part is very similar to BBa_K1362101 and BBa_K1362105 but provides a secondary Gate for the insertion of a protein or peptide of interest to the N-terminus of the intein. As no splicing occurs on that side of the Intein, the latter will stay with the intein after splicing. BBa_K1362106 is the complementary N-intein part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 826
Illegal AgeI site found at 938
Illegal AgeI site found at 2020
Illegal AgeI site found at 2132
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1115
Illegal BsaI site found at 2309
Illegal BsaI.rc site found at 40
Illegal BsaI.rc site found at 1234

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1362107 short</partinfo>
-This assembly construct is part of the iGEM team Heidelberg 2014's intein toolbox. It allows for easy generation of translational units of the Npu DnaE C-intein flanked by N and C terminal POIs as described in RFC[???].
+This assembly construct is part of the iGEM team Heidelberg 2014's intein toolbox. It allows for easy generation of translational units of the Npu DnaE C-intein flanked by N and C terminal POIs as described in RFC[105].
 This part is very similar to <partinfo>BBa_K1362101</partinfo> and <partinfo>BBa_K1362105</partinfo> but provides a secondary Gate for the insertion of a protein or peptide of interest to the N-terminus of the intein. As no splicing occurs on that side of the Intein, the latter will stay with the intein after splicing. <partinfo>BBa_K1362106</partinfo> is the complementary N-intein part.