Difference between revisions of "Part:BBa K1362058"
 
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PelB leader sequence. Fused to the N-terminus of a protein, it directs this protein to the E. coli periplasm.
 
PelB leader sequence. Fused to the N-terminus of a protein, it directs this protein to the E. coli periplasm.
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This part functions as an RFC[i] insert. It consists of (<partinfo>BBa_J32015</partinfo>) inserted into the carrier (<partinfo>BBa_K1362053</partinfo>).
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This part functions as an RFC[105] insert. It consists of (<partinfo>BBa_J32015</partinfo>) inserted into the carrier (<partinfo>BBa_K1362053</partinfo>).
 
===Usage and Biology===
 
===Usage and Biology===
  

Latest revision as of 16:07, 24 October 2014

PelB leading sequence as RFC[105] N-terminal insert

PelB leader sequence. Fused to the N-terminus of a protein, it directs this protein to the E. coli periplasm. This part functions as an RFC[105] insert. It consists of (BBa_J32015) inserted into the carrier (BBa_K1362053).

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 61
    Illegal NgoMIV site found at 71
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 97