Difference between revisions of "Part:BBa K1362055"
 
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The MinD MTS (membrane targeting sequence) from B. subtilis, when fused to the C-terminus of a protein, localizes it to the cytoplasmic face of the inner cell membrane in E. coli.  
 
The MinD MTS (membrane targeting sequence) from B. subtilis, when fused to the C-terminus of a protein, localizes it to the cytoplasmic face of the inner cell membrane in E. coli.  
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This part functions as an RFC[i] insert. It consists of (<partinfo>BBa_K1362255</partinfo>) inserted into the carrier (<partinfo>BBa_K1362052</partinfo>).
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This part functions as an RFC[105] insert. It consists of (<partinfo>BBa_K1362255</partinfo>) inserted into the carrier (<partinfo>BBa_K1362052</partinfo>).
  
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 16:05, 24 October 2014

MinD MTS as RFC[105] C-terminal insert

The MinD MTS (membrane targeting sequence) from B. subtilis, when fused to the C-terminus of a protein, localizes it to the cytoplasmic face of the inner cell membrane in E. coli. This part functions as an RFC[105] insert. It consists of (BBa_K1362255) inserted into the carrier (BBa_K1362052).

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 95
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 106
    Illegal SapI.rc site found at 44