Difference between revisions of "Part:BBa K1371041:Design"

 
Line 1: Line 1:
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1371041 short</partinfo>
 
<partinfo>BBa_K1371041 short</partinfo>
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===Source===
 
===Source===
  
−
aa
+
<i>Saccharopolyspora erythraea</i>
  
 
===References===
 
===References===
 +
[1]David E. Cane, Programming of Erythromycin Biosynthesis by a Modular Polyketide Synthase, J. Biol. Chem. (2010) 285:27517-27523.<p></p>
 +
[2]Blaine A. Pfeifer, Suzanne J. Admiraal, Hugo Gramajo, David E. Cane, Chaitan Khosla. Biosythesis of Complex Polyketides in a Metabolically Engineered Strain of, <i>E.coli</i> [J]. Science, 2001, 291(44): 1790-1792.<p></p>
 +
[3]Tae, Hongseok,Development of an Analysis Program of Type I Polyketide Synthase Gene Clusters Using Homology Search and Profile Hidden Markov Model, J. Microbiol. Biotechnol. (2009), 19(2), 140–146.<p></p>
 +
[4]Patrick Caffrey, Amphotericin biosynthesis in <i>Streptomyces</i> nodosus: deductions from analysis of polyketide synthase and late genes Chemistry & Biology 8 (2001) 713-723.<p></p>

Revision as of 16:01, 24 October 2014

RFP-DEBS1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2590
    Illegal BamHI site found at 7135
    Illegal BamHI site found at 9984
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1270
    Illegal NgoMIV site found at 2753
    Illegal NgoMIV site found at 3125
    Illegal NgoMIV site found at 3158
    Illegal NgoMIV site found at 3631
    Illegal NgoMIV site found at 4340
    Illegal NgoMIV site found at 5213
    Illegal AgeI site found at 604
    Illegal AgeI site found at 716
    Illegal AgeI site found at 1081
    Illegal AgeI site found at 2443
    Illegal AgeI site found at 2789
    Illegal AgeI site found at 3368
    Illegal AgeI site found at 5089
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2195
    Illegal BsaI.rc site found at 4625
    Illegal SapI.rc site found at 3460


Design Notes

aa


Source

Saccharopolyspora erythraea

References

[1]David E. Cane, Programming of Erythromycin Biosynthesis by a Modular Polyketide Synthase, J. Biol. Chem. (2010) 285:27517-27523.

[2]Blaine A. Pfeifer, Suzanne J. Admiraal, Hugo Gramajo, David E. Cane, Chaitan Khosla. Biosythesis of Complex Polyketides in a Metabolically Engineered Strain of, E.coli [J]. Science, 2001, 291(44): 1790-1792.

[3]Tae, Hongseok,Development of an Analysis Program of Type I Polyketide Synthase Gene Clusters Using Homology Search and Profile Hidden Markov Model, J. Microbiol. Biotechnol. (2009), 19(2), 140–146.

[4]Patrick Caffrey, Amphotericin biosynthesis in Streptomyces nodosus: deductions from analysis of polyketide synthase and late genes Chemistry & Biology 8 (2001) 713-723.