Difference between revisions of "Part:BBa K1362101:Design"
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===Source=== | ===Source=== | ||
| − | The part is assembled from different sources. The intein sequences come addgene or were kindly provided by different working groups. BBa_J04450 was used as mRFP selection marker. The scars and overhangs are part of the RFC[ | + | The part is assembled from different sources. The intein sequences come addgene or were kindly provided by different working groups. BBa_J04450 was used as mRFP selection marker. The scars and overhangs are part of the RFC[105] intein cloning strategy and are further explained on our [http://2014.igem.org/Team:Heidelberg/parts/rfc wiki] and in RFC[105] [[#Reference|[1]]]. Please see the corresponding basic parts for the individual detailed information. |
===References=== | ===References=== | ||
Latest revision as of 15:28, 24 October 2014
RBS+NpuDnaE C-intein RFC[105] assembly construct (with His6)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 958
Illegal AgeI site found at 1070 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1247
Illegal BsaI.rc site found at 172
Design Notes
???
Source
The part is assembled from different sources. The intein sequences come addgene or were kindly provided by different working groups. BBa_J04450 was used as mRFP selection marker. The scars and overhangs are part of the RFC[105] intein cloning strategy and are further explained on our [http://2014.igem.org/Team:Heidelberg/parts/rfc wiki] and in RFC[105] [1]. Please see the corresponding basic parts for the individual detailed information.