Difference between revisions of "Part:BBa K1493803"

Revision as of 14:31, 24 October 2014

promoter with CIλ and LacI operator sites

Promoter designed to have operators sites for the Lac repressor and the cI lambda repressor, based on sequences in Elowitz et. al. 2007

This promoter contains two LacI operator sites at the core and proximal position (middle and right part of the promoter) and two cI lambda operator sites at the distal site (left part of the promoter).

Usage and Biology

The promoter was combined with GFP to check for its functionality. Figure 1: plates with the six diferent promters, that where created for BananaGuard, upstream of GFP. P1 is BBa_K1493801, P2 is BBa_K1493802, P3 is BBa_K1493803, P4 is BBa_K1493804, P5 is BBa_K1493805, P6 is BBa_K1493806.

Figure 1 shows the P3 (promoter with CIλ and LacI operator sites), it is not expressing GFP suggesting that it is either non functional or a very weak promoter.

This Promoter is part of a promoter set used in [http://2014.igem.org/Team:Wageningen_UR BananaGuard] listed here:

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 Promoter designed to have operators sites for the Lac repressor and the cI lambda repressor, based on sequences in Elowitz et. al. 2007
-This Promoter is part of a promoter set used in BananaGuard listed here:
+This promoter contains two LacI operator sites at the core and proximal position (middle and right part of the promoter) and two cI lambda operator sites at the distal site (left part of the promoter).
+===Usage and Biology===
+The promoter was combined with [https://parts.igem.org/Part:BBa_I13504 GFP] to check for its functionality.
+https://static.igem.org/mediawiki/2014/f/f2/Wageningen_UR_killswitch_Pic13.jpg
+Figure 1: plates with the six diferent promters, that where created for BananaGuard, upstream of GFP. P1 is [https://parts.igem.org/Part:BBa_K1493801 BBa_K1493801], P2 is [https://parts.igem.org/Part:BBa_K1493802 BBa_K1493802], P3 is [https://parts.igem.org/Part:BBa_K1493803 BBa_K1493803], P4 is [https://parts.igem.org/Part:BBa_K1493804 BBa_K1493804], P5 is [https://parts.igem.org/Part:BBa_K1493805 BBa_K1493805], P6 is [https://parts.igem.org/Part:BBa_K1493806 BBa_K1493806].
+Figure 1 shows the P3 (promoter with CIλ and LacI operator sites), it is not expressing GFP suggesting that it is either non functional or a very weak promoter.
+This Promoter is part of a promoter set used in [http://2014.igem.org/Team:Wageningen_UR BananaGuard] listed here:
 [https://parts.igem.org/Part:BBa_K1493802 BBa_K1493801]
@@ Line 17: / Line 27: @@
 <!-- Add more about the biology of this part here
-===Usage and Biology===
 <!-- -->