Difference between revisions of "Part:BBa K1493801"

Revision as of 14:16, 24 October 2014

Promoter with Tet operator site

Promoter designed to have operators sites for the tetracycline repressor, based on sequences in Elowitz et. al. 2007

This promoter contains two TetR operator sites at the core and proximal position (middle and right part of the promoter)

The promoter was combined with GFP to check for its functionality. Figure 1: plates with the six diferent promters, that where created for BananaGuard, upstream of GFP. P1 is BBa_K1493801, P2 is BBa_K1493802, P3 is BBa_K1493803, P4 is BBa_K1493804, P5 is BBa_K1493805, P6 is BBa_K1493806.

Figure 1 shows the P1 (Promoter with Tet operator site) expressing GFP, suggesting that it is a functional promoter.

This Promoter is part of a promoter set used in BananaGuard listed here:

Sequence and Features

Assembly Compatibility:

@@ Line 3: / Line 3: @@
 Promoter designed to have operators sites for the tetracycline repressor, based on sequences in Elowitz et. al. 2007
+This promoter contains two TetR operator sites at the core and proximal position (middle and right part of the promoter)
+===Usage and Biology===
+The promoter was combined with [https://parts.igem.org/Part:BBa_I13504 GFP] to check for its functionality.
+https://static.igem.org/mediawiki/2014/f/f2/Wageningen_UR_killswitch_Pic13.jpg
+Figure 1: plates with the six diferent promters, that where created for BananaGuard, upstream of GFP. P1 is [https://parts.igem.org/Part:BBa_K1493801 BBa_K1493801], P2 is [https://parts.igem.org/Part:BBa_K1493802 BBa_K1493802], P3 is [https://parts.igem.org/Part:BBa_K1493803 BBa_K1493803], P4 is [https://parts.igem.org/Part:BBa_K1493804 BBa_K1493804], P5 is [https://parts.igem.org/Part:BBa_K1493805 BBa_K1493805], P6 is [https://parts.igem.org/Part:BBa_K1493806 BBa_K1493806].
+Figure 1 shows the P1 (Promoter with Tet operator site) expressing GFP, suggesting that it is a functional promoter.
 This Promoter is part of a promoter set used in BananaGuard listed here:
@@ Line 17: / Line 28: @@
 <!-- Add more about the biology of this part here
-===Usage and Biology===
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