Difference between revisions of "Part:BBa K1493560"
 
Line 4: Line 4:
  
 
L-Rhamnose inducable promoter witht the cI lambda represor combined with a PcI/tet with GFP.
 
L-Rhamnose inducable promoter witht the cI lambda represor combined with a PcI/tet with GFP.
 +
 +
When rhamnose is present the cI lambda repressor is expressed, repressing the pcI/Tet promoter and so inhibiting the expression of GFP.
  
 
This BioBrick is used by [http://2014.igem.org/Team:Wageningen_UR BananaGuard] to characterize the pcI/tet promoter with the [http://2014.igem.org/Team:Wageningen_UR/project/characterization rhamnose mediated characterization].
 
This BioBrick is used by [http://2014.igem.org/Team:Wageningen_UR BananaGuard] to characterize the pcI/tet promoter with the [http://2014.igem.org/Team:Wageningen_UR/project/characterization rhamnose mediated characterization].

Latest revision as of 13:30, 24 October 2014

pRHA+cIλ+pcI/tet+GFP


L-Rhamnose inducable promoter witht the cI lambda represor combined with a PcI/tet with GFP.

When rhamnose is present the cI lambda repressor is expressed, repressing the pcI/Tet promoter and so inhibiting the expression of GFP.

This BioBrick is used by [http://2014.igem.org/Team:Wageningen_UR BananaGuard] to characterize the pcI/tet promoter with the [http://2014.igem.org/Team:Wageningen_UR/project/characterization rhamnose mediated characterization].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1811