Difference between revisions of "Part:BBa M36268"

 
 
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<partinfo>BBa_M36268 short</partinfo>
 
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Quorum sensing is a system bacteria use to coordinate behaviors that correlate to cell density. Each species of bacteria produces and senses a unique, diffusable small molecule. This allows bacteria to "count" how many cells of their species are in the vicinity. Pseudomonas Aeruginosa, an opportunistic human pathogen associated with cystic fibrosis, uses two quorum sensing systems, with one of them being LasI/LasR. LasI produces N-(3-oxododecanoyl)-homoserine lactone, and LasR interacts with this molecule to transcriptionally regulate a variety of quorum sensing genes. One of these genes is qsc102. This composite part consists of a constitutive promoter, a RBS, the LasR gene, a terminator, and the qsc102 promoter. This entire construct is optimized for a plasmid that will be transformed into E. coli. This construct will be coupled to a standard fluorescence actuator so that in the presence of N-(3-oxododecanoyl)-homoserine lactone, the part will induce transcription of the fluorescence actuator.
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Quorum sensing is a system bacteria use to coordinate behaviors that correlate to cell density. Each species of bacteria produces and senses a unique, diffusable small molecule. This allows bacteria to "count" how many cells of their species are in the vicinity. Pseudomonas Aeruginosa, an opportunistic human pathogen associated with cystic fibrosis, uses two quorum sensing systems, with one of them being LasI/LasR. LasI produces N-(3-oxododecanoyl)-homoserine lactone, and LasR interacts with this molecule to transcriptionally regulate a variety of quorum sensing genes. One of these genes is qsc102.
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This composite part consists of a constitutive promoter (BBa_M36266), a RBS (BBa_M36269), the LasR gene (BBa_M36264), a terminator (BBa_M36135), and the qsc102 promoter (BBa_M36265). This entire construct is optimized for a plasmid that will be transformed into E. coli.  
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===Usage and Biology===
 
===Usage and Biology===
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This construct will be coupled to a standard fluorescence actuator so that in the presence of N-(3-oxododecanoyl)-homoserine lactone, the part will induce transcription of the fluorescence actuator. Furthermore, this composite part will be used in conjunction with a LasI actuator: BBa_M36263. LasI will produce the N-(3-oxododecanoyl)-homoserine lactone that will be used with the LasR sensor.
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_M36268 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_M36268 SequenceAndFeatures</partinfo>

Latest revision as of 06:34, 24 October 2014

LasR-N-(3-oxododecanoyl)-homoserine lactone qsc102 sensor

Quorum sensing is a system bacteria use to coordinate behaviors that correlate to cell density. Each species of bacteria produces and senses a unique, diffusable small molecule. This allows bacteria to "count" how many cells of their species are in the vicinity. Pseudomonas Aeruginosa, an opportunistic human pathogen associated with cystic fibrosis, uses two quorum sensing systems, with one of them being LasI/LasR. LasI produces N-(3-oxododecanoyl)-homoserine lactone, and LasR interacts with this molecule to transcriptionally regulate a variety of quorum sensing genes. One of these genes is qsc102.

This composite part consists of a constitutive promoter (BBa_M36266), a RBS (BBa_M36269), the LasR gene (BBa_M36264), a terminator (BBa_M36135), and the qsc102 promoter (BBa_M36265). This entire construct is optimized for a plasmid that will be transformed into E. coli.


Usage and Biology

This construct will be coupled to a standard fluorescence actuator so that in the presence of N-(3-oxododecanoyl)-homoserine lactone, the part will induce transcription of the fluorescence actuator. Furthermore, this composite part will be used in conjunction with a LasI actuator: BBa_M36263. LasI will produce the N-(3-oxododecanoyl)-homoserine lactone that will be used with the LasR sensor.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 360
    Illegal PstI site found at 129
    Illegal PstI site found at 537
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 360
    Illegal NheI site found at 141
    Illegal PstI site found at 129
    Illegal PstI site found at 537
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 360
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 360
    Illegal PstI site found at 129
    Illegal PstI site found at 537
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 360
    Illegal PstI site found at 129
    Illegal PstI site found at 537
    Illegal AgeI site found at 581
  • 1000
    COMPATIBLE WITH RFC[1000]