Difference between revisions of "Part:BBa K1440066"
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<partinfo>BBa_K1440066 short</partinfo> | <partinfo>BBa_K1440066 short</partinfo> | ||
− | + | this part consists of reverse CMV and reverse RFP and tet-on shRFP system. With the transfection of this plasmid into the cell lines, RFP will be expressed constitutely. if dox is dosed with the cell lines, shRFP will be expressed, then knockdown the RFP expression.It must be used in mammalian cells. | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 03:04, 24 October 2014
reverse CMV->reverse RFP->pTRE->shRNA
this part consists of reverse CMV and reverse RFP and tet-on shRFP system. With the transfection of this plasmid into the cell lines, RFP will be expressed constitutely. if dox is dosed with the cell lines, shRFP will be expressed, then knockdown the RFP expression.It must be used in mammalian cells.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1184
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1184
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1184
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1184
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1184
Illegal AgeI site found at 13
Illegal AgeI site found at 125
Illegal AgeI site found at 1116 - 1000COMPATIBLE WITH RFC[1000]