Difference between revisions of "Part:BBa K1456003"
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<partinfo>BBa_K1456003 short</partinfo>
 
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<center>[[File:Atoms sod 6.png|400px|]]</center><br/>
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<li>Transfected Tet off - pTRE and Tet off - pTRE-SOD1 vectors were subjected to SOD assay and measured using the spectrophotometer at 560 nm. Cells which have the Tet off – pTRE SOD1 gene present can degrade the oxygen radicals 21 times faster. SOD assay was performed for the proteins obtained from the transfected SOD1 visible colour change could be seen.
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<center>[[File:Atoms sod 7.png|200px|]]</center><br/>
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<center>https://static.igem.org/mediawiki/2014/thumb/4/40/ATOMS-Biobricks-XO-SOD-GPx-Aprotinin.jpg/800px-ATOMS-Biobricks-XO-SOD-GPx-Aprotinin.jpg</center>
 
<center>https://static.igem.org/mediawiki/2014/thumb/4/40/ATOMS-Biobricks-XO-SOD-GPx-Aprotinin.jpg/800px-ATOMS-Biobricks-XO-SOD-GPx-Aprotinin.jpg</center>
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<center>[[File:Atoms sod 6.png|400px|]]</center><br/>
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<li>Transfected Tet off - pTRE and Tet off - pTRE-SOD1 vectors were subjected to SOD assay and measured using the spectrophotometer at 560 nm. Cells which have the Tet off – pTRE SOD1 gene present can degrade the oxygen radicals 21 times faster. SOD assay was performed for the proteins obtained from the transfected SOD1 visible colour change could be seen.
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<center>[[File:Atoms sod 7.png|200px|]]</center><br/>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 15:32, 23 October 2014

Superoxide dismutase-1 (SOD-1)


  • SOD is a powerful and essential antioxidant enzyme which converts and scavenges free radicals and reactive oxygen species (ROS) into hydrogen peroxide (H2O2), slightly less harmful compound in order to convey it to the second step reaction which detoxifies H2O2 into water. (Batelli et al. 1972; Baudry et al. 1993; Gonzalez et al. 1995; Doctrow et al. 1996)
  • In our body cells, there are three different subtypes of SOD enzyme in different cellular levels. SOD-1 exists in cytoplasm, SOD-2 in mitochondria and SOD-3 is in extracellular matrix. We have chosen SOD-1 to take advantage of easy interference and activity capacity due to its location. Additionally, there is evidence in the literature for the synthetic production of SOD-1 enzyme by transfecting human cells. (Shuvaev et al. 2013)
800px-ATOMS-Biobricks-XO-SOD-GPx-Aprotinin.jpg
Atoms sod 6.png

  • Transfected Tet off - pTRE and Tet off - pTRE-SOD1 vectors were subjected to SOD assay and measured using the spectrophotometer at 560 nm. Cells which have the Tet off – pTRE SOD1 gene present can degrade the oxygen radicals 21 times faster. SOD assay was performed for the proteins obtained from the transfected SOD1 visible colour change could be seen.
    Atoms sod 7.png

    Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      COMPATIBLE WITH RFC[12]
    • 21
      COMPATIBLE WITH RFC[21]
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      COMPATIBLE WITH RFC[25]
    • 1000
      INCOMPATIBLE WITH RFC[1000]
      Illegal BsaI.rc site found at 327