Difference between revisions of "Part:BBa K1493701"
 
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<partinfo>BBa_K1493701 short</partinfo>
 
<partinfo>BBa_K1493701 short</partinfo>
  
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This part was designed for the construction of a CI repressor inducible toggle switch by BananaGuard. Combined with pTet + LacI it would form a toggle switch expressing GFP in the state in which also TetR is produced. Further experiments with this part were discontinued  and a toggle switch with GFP downstream of pTet + LacI was constructed. Information of that part can be found on <partinfo>BBa_K1493702</partinfo> and <partinfo>BBa_K1493703</partinfo>.  
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This part was designed for the construction of a CI repressor inducible toggle switch by [http://2014.igem.org/Team:Wageningen_UR/project/kill-switch#toggleswitchplasmid BananaGuard]. Combined with pTet + LacI it would form a toggle switch expressing GFP in the state in which also TetR is produced. Further experiments with this part were discontinued  and a toggle switch with GFP downstream of pTet + LacI was constructed. Information of that part can be found on <partinfo>BBa_K1493702</partinfo> and <partinfo>BBa_K1493703</partinfo>.  
  
  

Latest revision as of 14:55, 23 October 2014

pCI/Lac + TetR + GFP

This part was designed for the construction of a CI repressor inducible toggle switch by [http://2014.igem.org/Team:Wageningen_UR/project/kill-switch#toggleswitchplasmid BananaGuard]. Combined with pTet + LacI it would form a toggle switch expressing GFP in the state in which also TetR is produced. Further experiments with this part were discontinued and a toggle switch with GFP downstream of pTet + LacI was constructed. Information of that part can be found on BBa_K1493702 and BBa_K1493703.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1589