Difference between revisions of "Part:BBa K1431814:Experience"
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Latest revision as of 13:06, 23 October 2014

Applications of BBa_K1431814

After we constructed this part, we tranformed into BL21 & DH5α to test expression effeciency. In the meantime, we did a comparison between BBa_K1431814 and BBa_K1431834 whose promoter is BBa_J23106, a relative weak promoter.

LB agar plate growth comparison

We spread the plate for BL21 at 10:00am on 15th, Oct and DH5α at 11:00am on 15th, Oct. The plate contains 34ug/ml chloramphenicol. Following pictures were taken every several hours after placing the plates into a 37℃ incubator.

JA is J23100,JB is J23106.

At 00:30 (16th,Oct.)

SUSTC-34_916-1.png


At 1:50 (16th,Oct.)

SUSTC-34_916-2.png


At 3:00 (16th,Oct.)

SUSTC-34_916-3.png


At 6:00 (16th,Oct.)

SUSTC_34_916-4.png


At 7:40 (16th,Oct.)

SUSTC_34_916-5.png


At 8:00 (16th,Oct.)

SUSTC_34_916-6.png


At 10:00 (16th,Oct.)

SUSTC_34_916-7.png


At 11:20 (16th,Oct.)

SUSTC_34_916-8.png

LB broth growth comparison

We isolated single colonies from the plates and incubate in 2ml LB broth with 34ug/ml chloramphenicol. Then we isolated single colonies from the plates.

At 00:50 (17th,Oct.)

SUSTC_34_916-1.png.jpg


At 02:45 (17th,Oct.)

SUSTC_34_916-2.png.jpg


At 04:55 (17th,Oct.)

SUSTC_34_916-3.jpg


At 10:40 (17th,Oct.)

SUSTC_34_916-4.jpg

Conclusion

With observation, BBa_K1431814 is weaker than BBa_K1431834 unexpectedly.

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