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The measurements in Figure 1 indicate a tuneable activation of pRha by rhamnose. The RFU values of 0% and 0.001% rhamnose are not significant taking into account the high standard deviation for these measurements as can be seen in Figure 1B. From a rhamnose concentration of 0.01% to 0.2% a significant increase in fluorescence is measured. Fluorescence from Figure 2 can be used to predict the concentration of repressor protein produced. Data points for time 8.25 h were chosen for the graph in Figure 2B due to the peak at time for 0.2% rhamnose, which is visible in Figure 1. This peak can be explained by the rhamnose depletion, as it is consumed by E. coli, causing the stop of promoter induction. We conclude that the L-rhamnose inducible promoter part BBa_K914003 is a well functional part. | The measurements in Figure 1 indicate a tuneable activation of pRha by rhamnose. The RFU values of 0% and 0.001% rhamnose are not significant taking into account the high standard deviation for these measurements as can be seen in Figure 1B. From a rhamnose concentration of 0.01% to 0.2% a significant increase in fluorescence is measured. Fluorescence from Figure 2 can be used to predict the concentration of repressor protein produced. Data points for time 8.25 h were chosen for the graph in Figure 2B due to the peak at time for 0.2% rhamnose, which is visible in Figure 1. This peak can be explained by the rhamnose depletion, as it is consumed by E. coli, causing the stop of promoter induction. We conclude that the L-rhamnose inducible promoter part BBa_K914003 is a well functional part. | ||
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Revision as of 08:50, 23 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K914003
User Reviews
UNIQ841ec0bed055a6ac-partinfo-00000000-QINU
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No review score entered. Wageningen UR 2014 |
We used this part in our project and characetrized it using gfp.
Figure 1. Average GFP fluorescence of E. coli DH5α carrying a pSB3K3 plasmid containing the pRha gfp BioBrick. At t=0 the cells were induced with different concentrations of rhamnose in triplo. Fluorescence is measured in a plate reader in time (A) and at time point 8.25 (B) normalized for OD600. The measurements in Figure 1 indicate a tuneable activation of pRha by rhamnose. The RFU values of 0% and 0.001% rhamnose are not significant taking into account the high standard deviation for these measurements as can be seen in Figure 1B. From a rhamnose concentration of 0.01% to 0.2% a significant increase in fluorescence is measured. Fluorescence from Figure 2 can be used to predict the concentration of repressor protein produced. Data points for time 8.25 h were chosen for the graph in Figure 2B due to the peak at time for 0.2% rhamnose, which is visible in Figure 1. This peak can be explained by the rhamnose depletion, as it is consumed by E. coli, causing the stop of promoter induction. We conclude that the L-rhamnose inducible promoter part BBa_K914003 is a well functional part. |
UNIQ841ec0bed055a6ac-partinfo-00000002-QINU