Difference between revisions of "Part:BBa K1321335:Experience"
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===Applications of BBa_K1321335=== | ===Applications of BBa_K1321335=== | ||
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| + | Part BBa_K1321335 was digested with XbaI and PstI and the resulting fragment, containing the optimised coding sequences of AcsC and AcsD, was cloned into a medium-to-low copy number plasmid (pSB3K3) containing the inducible pLAC promoter. The resulting construct, verified by GreenTaq Colony PCR, was subsequently sub-cloned into BBa_K1321336-containing electrocompetent cells. For more information on the characterisation procedure, click here: https://parts.igem.org/Part:BBa_K1321334:Experience | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 07:18, 23 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1321335
Part BBa_K1321335 was digested with XbaI and PstI and the resulting fragment, containing the optimised coding sequences of AcsC and AcsD, was cloned into a medium-to-low copy number plasmid (pSB3K3) containing the inducible pLAC promoter. The resulting construct, verified by GreenTaq Colony PCR, was subsequently sub-cloned into BBa_K1321336-containing electrocompetent cells. For more information on the characterisation procedure, click here: https://parts.igem.org/Part:BBa_K1321334:Experience
User Reviews
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