Difference between revisions of "Part:BBa K1321335"

Revision as of 07:12, 23 October 2014

acsCD

This part contains the coding sequence, previously codon-optimised for expression in Escherichia coli, of the Cellulose Synthesis elements AcsC and AcsD. In Gluconacetobacter xylinus, the Acs cellulose synthesis operon is a functional unit that codes for four essential elements for cellulose assembly and release into the extracellular space. Even though the functions of AcsC and AcsD are still not very well understood and very little is known about the assembly, localisation and activity of this biosynthetic process, these proteins are required for optimal production yields. AcsC is believed to code for an oligomeric protein that becomes embedded in the extracellular membrane for further pore formation and subsequent release of the growing polymer into the extracellular space. On the other hand, AcsD improves yields by optimising crystallisation, but the function and localisation of this element have not yet been fully determined.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1837
Illegal BglII site found at 4382
Illegal BamHI site found at 1906
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 778
Illegal AgeI site found at 1334
Illegal AgeI site found at 1913
Illegal AgeI site found at 2123
Illegal AgeI site found at 2975
Illegal AgeI site found at 3293
Illegal AgeI site found at 3638
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1321335 short</partinfo>
-This part contains the coding sequence, previously codon-optimised for expression in Escherichia coli, of the Cellulose Synthesis elements AcsC and AcsD. In Gluconacetobacter xylinus, the Acs cellulose synthesis operon is a functional unit that codes for four essential elements for cellulose assembly and release into the extracellular space. Even though the functions of AcsC and AcsD are still not very well understood and very little is known about the assembly, localisation and activity of this biosynthetic process, these proteins are required for optimal production yields. AcsC is believed to code for an oligomeric protein that becomes embedded in the extracellular membrane for further pore formation and subsequent release of the growing polymer into the extracellular space. On the other hand, AcsD improves yields by optimising crystallisation, but the function and localisation of this element have not yet been fully determined.
+This part contains the coding sequence, previously codon-optimised for expression in <em>Escherichia col</em>i, of the Cellulose Synthesis elements AcsC and AcsD. In <em>Gluconacetobacter xylinus</em>, the Acs cellulose synthesis operon is a functional unit that codes for four essential elements for cellulose assembly and release into the extracellular space. Even though the functions of AcsC and AcsD are still not very well understood and very little is known about the assembly, localisation and activity of this biosynthetic process, these proteins are required for optimal production yields. AcsC is believed to code for an oligomeric protein that becomes embedded in the extracellular membrane for further pore formation and subsequent release of the growing polymer into the extracellular space. On the other hand, AcsD improves yields by optimising crystallisation, but the function and localisation of this element have not yet been fully determined.
 <!-- Add more about the biology of this part here