Difference between revisions of "Part:BBa K1440000:Experience"
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===Applications of BBa_K1440000===
 
===Applications of BBa_K1440000===
 
   This part is used to test the cre-TM system and pTRE system. In this part, although we use mCFP as the reporter gene, we find that the efficiency of mCFP is not quite good, so we replace the mCFP reporter gene with the EGFP to test the cre-TM system. And because it is a big part, so we also replace the mCFP with OSKM(four transcription factors) binding with T2A linkers. We will only show the result with EGFP and OSKM.
 
   This part is used to test the cre-TM system and pTRE system. In this part, although we use mCFP as the reporter gene, we find that the efficiency of mCFP is not quite good, so we replace the mCFP reporter gene with the EGFP to test the cre-TM system. And because it is a big part, so we also replace the mCFP with OSKM(four transcription factors) binding with T2A linkers. We will only show the result with EGFP and OSKM.
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===User Reviews===
 
===User Reviews===
 
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<!-- DON'T DELETE --><partinfo>BBa_K1440000 StartReviews</partinfo>

Revision as of 06:10, 23 October 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1440000

 This part is used to test the cre-TM system and pTRE system. In this part, although we use mCFP as the reporter gene, we find that the efficiency of mCFP is not quite good, so we replace the mCFP reporter gene with the EGFP to test the cre-TM system. And because it is a big part, so we also replace the mCFP with OSKM(four transcription factors) binding with T2A linkers. We will only show the result with EGFP and OSKM.

User Reviews

UNIQafd23865d9c55c05-partinfo-00000000-QINU UNIQafd23865d9c55c05-partinfo-00000001-QINU