Difference between revisions of "Part:BBa K1326004"
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Magnesium chelatase subunit I ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080000 ChlI1]) and Magnesium chelatase subunit D ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080002 ChlD]) form an ATP-dependent complex (increased yield is seen if [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080011 ChlI2] subunit is also present). This complex acts on protoporphyrin IX, bound to the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080001 ChlH] protein, and inserts a magnesium ion which produces Mg-protoporphyrin IX. | Magnesium chelatase subunit I ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080000 ChlI1]) and Magnesium chelatase subunit D ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080002 ChlD]) form an ATP-dependent complex (increased yield is seen if [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080011 ChlI2] subunit is also present). This complex acts on protoporphyrin IX, bound to the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080001 ChlH] protein, and inserts a magnesium ion which produces Mg-protoporphyrin IX. | ||
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| + | ===Usage and Biology=== | ||
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Has since been added to: [https://parts.igem.org/Part:BBa_K1326008 BBa_K1326008] | Has since been added to: [https://parts.igem.org/Part:BBa_K1326008 BBa_K1326008] | ||
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| + | This composite part was used to add further parts on to to form a functional operon. The two genes present have been found to be functional, converting Protoporphyrin IX into Mg-protoporphyrin IX. These results can bee seen below in Functional Parameters. | ||
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Latest revision as of 02:56, 23 October 2014
Plac + ChlI1 + ChlD
Composite part incorporating the genes adding a Mg ion to protoporphyrin IX, as a part of the chlorophyll biosynthetic pathway.
Magnesium chelatase subunit I (ChlI1) and Magnesium chelatase subunit D (ChlD) form an ATP-dependent complex (increased yield is seen if ChlI2 subunit is also present). This complex acts on protoporphyrin IX, bound to the ChlH protein, and inserts a magnesium ion which produces Mg-protoporphyrin IX.
Usage and Biology
Has since been added to: BBa_K1326008
This composite part was used to add further parts on to to form a functional operon. The two genes present have been found to be functional, converting Protoporphyrin IX into Mg-protoporphyrin IX. These results can bee seen below in Functional Parameters.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 2666
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 439
Illegal BglII site found at 1095
Illegal BglII site found at 1290
Illegal BglII site found at 3371
Illegal BamHI site found at 487 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1946
Illegal NgoMIV site found at 2765
Illegal NgoMIV site found at 3307 - 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
Figure 1: Fluorescence spectra at 420 nm excitation of induced ChlI1+ChlD extract following incubation in Figure 1. Peak observed at 595nm is consistent with Mg-Protoporphyrin IX production, demonstrating significant activity of the submitted BioBrick part.
Figure 2: Increasing fluorescence intensity over time through function of /ChlI1 + ChlD/ composite part..