Difference between revisions of "Part:BBa K1326008"
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| − | Figure 2: Single and double restriction digests of Operon 1. Single digest shows total plasmid length of 7.59 kB. Double digest shows Operon 1 fragment length of 4.39 kB, and BioBrick plasmid backbone length at 2.20 kB. | + | <b>''Figure 2:''</b> Single and double restriction digests of Operon 1. Single digest shows total plasmid length of 7.59 kB. Double digest shows Operon 1 fragment length of 4.39 kB, and BioBrick plasmid backbone length at 2.20 kB. |
Latest revision as of 23:58, 22 October 2014
Plac + ChlI1 + ChlD + GUN4
Composite part comprised of genes encoding Magnesium chelatase subunits: ChlI1, ChlD, and GUN4.
Figure 1: Operon 1 in the chlorophyll biosynthesis pathway.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 2666
Illegal NotI site found at 4213 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 439
Illegal BglII site found at 1095
Illegal BglII site found at 1290
Illegal BglII site found at 3371
Illegal BglII site found at 3898
Illegal BamHI site found at 487 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1946
Illegal NgoMIV site found at 2765
Illegal NgoMIV site found at 3307 - 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
Figure 2: Single and double restriction digests of Operon 1. Single digest shows total plasmid length of 7.59 kB. Double digest shows Operon 1 fragment length of 4.39 kB, and BioBrick plasmid backbone length at 2.20 kB.