Difference between revisions of "Part:BBa K1330000:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1330000 short</partinfo> | <partinfo>BBa_K1330000 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The sequence was synthesised, and amplified using High-Fidelity PCR. | |
| + | The PCR product was cut and ligated with pSB1C3. | ||
| + | |||
| + | The fusion with tRNA was designed as described in (tRNA article). | ||
| + | The Spinach2 sequence comes from (Spinach2 article). This BioBrick is a modified version of Spinach2 that has been modified (link to parts page) to remove an internal SpeI site. Spinach2.1 has been confirmed to function equally well as Spinach2. | ||
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
Revision as of 16:33, 22 October 2014
Spinach2.1 flanked by tRNALys3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was synthesised, and amplified using High-Fidelity PCR. The PCR product was cut and ligated with pSB1C3.
The fusion with tRNA was designed as described in (tRNA article). The Spinach2 sequence comes from (Spinach2 article). This BioBrick is a modified version of Spinach2 that has been modified (link to parts page) to remove an internal SpeI site. Spinach2.1 has been confirmed to function equally well as Spinach2.