Difference between revisions of "Part:BBa K1555007:Design"
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===Source=== | ===Source=== | ||
| − | + | 20nt target sequence was synthesized artificially | |
| + | |||
| + | the scaffold was acquired by PCR from a plasimid made by CAS | ||
===References=== | ===References=== | ||
Latest revision as of 06:40, 22 October 2014
copA's 20nt target sequence+sgRNA scaffold
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used overlap PCR to link 20nt target sequence with sgRNA scaffold.
Source
20nt target sequence was synthesized artificially
the scaffold was acquired by PCR from a plasimid made by CAS