Difference between revisions of "Part:BBa K1323000"

Revision as of 01:40, 22 October 2014

sgRNA (1): CRISPR Silencing of YFP

Single guide RNA (sgRNA) 1 is an RNA oligo that associates with dCas9 BBa_K1323002 and guides the CRISPRi complex to the coding strand of the YFP BBa_K1323010 at nt position 43 - 62. sgRNA is a fusion of crRNA (CRISPR RNA) and tracrRNA (trans-activation crRNA) - the 20 bp sequence is complementary to the target sequence and is linked to a hairpin loop that is similar to tracrRNA. When the sgRNA-dCas9 complex binds to the target sequence, it is expected to act as a programmable repressor and silence expression of the target gene.

This part was DNA-synthesized by Bio Basic.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1323000 short</partinfo>
-Single guide RNA (sgRNA) 1 is an RNA oligo that associates with dCAS9 [https://parts.igem.org/Part:BBa_K1323002 BBa_K1323002] and guides the CRISPRi complex to the coding strand of the YFP [https://parts.igem.org/Part:BBa_K1323010 BBa_K1323010] at nt position 43 - 62. sgRNA is a fusion of tracrRNA (trans-activation crRNA) and crRNA (CRISPR RNA) - the 20 bp sequence is complementary to the target sequence and is linked to a hairpin loop that is similar to tracrRNA. When the sgRNA-dCas9 complex binds to the target sequence, it is expected to act as a programmable repressor and silence expression of the target gene.
+Single guide RNA (sgRNA) 1 is an RNA oligo that associates with dCas9 [https://parts.igem.org/Part:BBa_K1323002 BBa_K1323002] and guides the CRISPRi complex to the coding strand of the YFP [https://parts.igem.org/Part:BBa_K1323010 BBa_K1323010] at nt position 43 - 62. sgRNA is a fusion of crRNA (CRISPR RNA) and tracrRNA (trans-activation crRNA) - the 20 bp sequence is complementary to the target sequence and is linked to a hairpin loop that is similar to tracrRNA. When the sgRNA-dCas9 complex binds to the target sequence, it is expected to act as a programmable repressor and silence expression of the target gene.
 This part was DNA-synthesized by Bio Basic.