Difference between revisions of "Part:BBa K747096:Experience"
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In order to measure its expression, we culture transformed E.coli in agar plates and measured the fluorescense intensity every 45 minutes. After that, we obtained the following data. [Fig.1] | In order to measure its expression, we culture transformed E.coli in agar plates and measured the fluorescense intensity every 45 minutes. After that, we obtained the following data. [Fig.1] | ||
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Revision as of 01:29, 22 October 2014
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K747096
Characterization by BIOSINT_Mexico iGEM 2014
CMV promoter fused with YFP by BIOSINT_Mexico iGEM 2014
As a part of the project from 2014_BIOSINT_Mexico , we fused the promoter PCMV (BBa_K747096) to a YFP reporter (BBa_E0030).
In order to measure its expression, we culture transformed E.coli in agar plates and measured the fluorescense intensity every 45 minutes. After that, we obtained the following data. [Fig.1]
We made three repetitions, so in order to model the equation, we obtained the mean measure, therefore:
We analysed the data and using the Wolfram Mathematica software, we obtained the following equation that fits the data.
If we plot this equation, we obtain:
Where we can see that the intensity of YFP grows directly proportional to the time and concentration of the molecule.
User Reviews
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