Difference between revisions of "Part:BBa K747096:Experience"
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===Applications of BBa_K747096=== | ===Applications of BBa_K747096=== | ||
| + | ==Characterization by BIOSINT_Mexico iGEM 2014== | ||
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| + | ===CMV promoter fused with YFP by BIOSINT_Mexico iGEM 2014=== | ||
As a part of the project from 2014_BIOSINT_Mexico , we fused the promoter PCMV (BBa_K747096) to a YFP reporter (BBa_E0030). | As a part of the project from 2014_BIOSINT_Mexico , we fused the promoter PCMV (BBa_K747096) to a YFP reporter (BBa_E0030). | ||
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::::::[[File:1res.png|600px|left|center|'''Figure 3''' Intensity YFP vs time.]] | ::::::[[File:1res.png|600px|left|center|'''Figure 3''' Intensity YFP vs time.]] | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 01:19, 22 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K747096
Characterization by BIOSINT_Mexico iGEM 2014
CMV promoter fused with YFP by BIOSINT_Mexico iGEM 2014
As a part of the project from 2014_BIOSINT_Mexico , we fused the promoter PCMV (BBa_K747096) to a YFP reporter (BBa_E0030).
In order to measure its expression, we culture transformed E.coli in agar plates and measured the fluorescense intensity every 45 minutes. After that, we obtained the following data. [Fig.1]
We made three repetitions, so in order to model the equation, we obtained the mean measure, therefore:
We analysed the data and using the Wolfram Mathematica software, we obtained the following equation that fits the data.
If we plot this equation, we obtain:
Where we can see that the intensity of YFP grows directly proportional to the time and concentration of the molecule.
User Reviews
UNIQ7eda1d85efb060da-partinfo-00000000-QINU UNIQ7eda1d85efb060da-partinfo-00000001-QINU