Difference between revisions of "Part:BBa K1323014"
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This sequence contains the T5X xylose inducible promoter and the xylose promoter repressor, XylR, for ''Staphylococcus''. It has been shown by Forsyth et al. (2002) that 2% xylose in LB is sufficient to generate significant expression downstream of this promoter (Forsyth et al, 2002). In the absence of xylose, XylR represses activity by binding to the promoter region. Furthermore, glucose was shown to repress the activity of this promoter (Wieland et al., 1995). | This sequence contains the T5X xylose inducible promoter and the xylose promoter repressor, XylR, for ''Staphylococcus''. It has been shown by Forsyth et al. (2002) that 2% xylose in LB is sufficient to generate significant expression downstream of this promoter (Forsyth et al, 2002). In the absence of xylose, XylR represses activity by binding to the promoter region. Furthermore, glucose was shown to repress the activity of this promoter (Wieland et al., 1995). | ||
| − | Our team obtained the sequence for this part from Dr. Donegan from the Ambrose Lab at The Geisel School of Medicine, Dartmouth. This part was synthesized | + | |
| + | Our team obtained the sequence for this part from Dr. Donegan from the Ambrose Lab at The Geisel School of Medicine, Dartmouth. This part was synthesized by Bio Basic. By doing so, we were able to remove 3 illegal sites. | ||
Latest revision as of 01:18, 22 October 2014
Xylose Inducible Promoter for Staphylococcus aureus
This sequence contains the T5X xylose inducible promoter and the xylose promoter repressor, XylR, for Staphylococcus. It has been shown by Forsyth et al. (2002) that 2% xylose in LB is sufficient to generate significant expression downstream of this promoter (Forsyth et al, 2002). In the absence of xylose, XylR represses activity by binding to the promoter region. Furthermore, glucose was shown to repress the activity of this promoter (Wieland et al., 1995).
Our team obtained the sequence for this part from Dr. Donegan from the Ambrose Lab at The Geisel School of Medicine, Dartmouth. This part was synthesized by Bio Basic. By doing so, we were able to remove 3 illegal sites.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1476
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Forsyth RA, et al., 2002. A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol Microbiol 43:1387–1400.
Wieland, K.P, Wieland, B. Gotz, F. 1995. A promoter screening plasmid and xylose inducible glucose-repressible expression vectors for Staphylococcus carnosus. Gene. 158: 91- 96.