Difference between revisions of "Part:BBa K1431813"

(→‎Selective Figures)
(→‎Selective Figures)
Line 8: Line 8:
 
===Selective Figures===
 
===Selective Figures===
 
<center>https://static.igem.org/mediawiki/parts/0/04/K11_BL21.jpg</center>
 
<center>https://static.igem.org/mediawiki/parts/0/04/K11_BL21.jpg</center>
−
<center>'''LB agar plate of Biobricks BBa_K1431812 transformed in BL21 after incubating 23h at 37℃(left)'''</center>
+
<center>'''LB agar plate of Biobricks BBa_K1431813 transformed in BL21 after incubating 23h at 37℃(left)'''</center>
  
  
 
<center>https://static.igem.org/mediawiki/parts/0/0a/K11_dh.jpg</center>
 
<center>https://static.igem.org/mediawiki/parts/0/0a/K11_dh.jpg</center>
−
<center>'''LB agar plate of Biobricks BBa_K1431812 transformed in DH5α after incubating 23h at 37℃(left)'''</center>
+
<center>'''LB agar plate of Biobricks BBa_K1431813 transformed in DH5α after incubating 23h at 37℃(left)'''</center>
  
 
==='''There is something wrong that bacteria didn't grow on plates.'''===
 
==='''There is something wrong that bacteria didn't grow on plates.'''===

Revision as of 01:12, 22 October 2014

cjBlue, green chromoprotein reporter system (Strong Promoter, Strong RBS)

Team Uppsala 2012 chromoprotein attracts many interests because its more convenient than fluorescent protein to be use as a reporter. However, few characterized data can be found for these proteins. SUSTC-Shenzhen this year want to tackle with theses problems.

We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene.

Selective Figures

K11_BL21.jpg
LB agar plate of Biobricks BBa_K1431813 transformed in BL21 after incubating 23h at 37℃(left)


K11_dh.jpg
LB agar plate of Biobricks BBa_K1431813 transformed in DH5α after incubating 23h at 37℃(left)

There is something wrong that bacteria didn't grow on plates.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]