Difference between revisions of "Part:BBa K747096:Experience"

(→‎User Reviews)
(→‎Applications of BBa_K747096)
Line 5: Line 5:
  
 
===Applications of BBa_K747096===
 
===Applications of BBa_K747096===
 +
 +
As part of the iGEM team BIOSINT_Mexico for 2014, e fused the promoter PCMV (BBa_K747096) to a YFP reporter (BBa_E0030).
 +
 +
In order to measure its expression, we culture transformed E.coli in agar plates and measured the fluorescense intensity every 45 minutes. After that, we obtained the following data. [Fig.1]
 +
 +
:::[[File:1res.png|150px|thumb|left|'''Figure 1''' YFP intensity every 45 minutes.]]
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
We made three repetitions, so in order to model the equation, we obtained the mean measure, therefore:
 +
 +
 +
 +
::::::[[File:2res.png|300px|thumb|left|'''Figure 2''' Media YFP intensity every 45 minutes.]]
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
We analysed the data and using the Wolfram Mathematica software, we obtained the following equation that fits the data.
 +
If we plot this equation, we obtain:
 +
 +
[[File:Model1res.png|400px|center|]]
 +
 +
Where we can see that the intensity of YFP grows directly proportional to the time and concentration of the molecule.
 +
 +
::::::[[File:4res.png|600px|left|center|'''Figure 3''' Intensity YFP vs time.]]
  
 
===User Reviews===
 
===User Reviews===

Revision as of 01:02, 22 October 2014


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K747096

As part of the iGEM team BIOSINT_Mexico for 2014, e fused the promoter PCMV (BBa_K747096) to a YFP reporter (BBa_E0030).

In order to measure its expression, we culture transformed E.coli in agar plates and measured the fluorescense intensity every 45 minutes. After that, we obtained the following data. [Fig.1]

Figure 1 YFP intensity every 45 minutes.














We made three repetitions, so in order to model the equation, we obtained the mean measure, therefore:


Figure 2 Media YFP intensity every 45 minutes.






We analysed the data and using the Wolfram Mathematica software, we obtained the following equation that fits the data. If we plot this equation, we obtain:

Model1res.png

Where we can see that the intensity of YFP grows directly proportional to the time and concentration of the molecule.

Figure 3 Intensity YFP vs time.

User Reviews

UNIQ6495a4a6d0bec0cd-partinfo-00000000-QINU UNIQ6495a4a6d0bec0cd-partinfo-00000001-QINU