Difference between revisions of "Part:BBa K1429001"
 
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===Usage and Biology===
 
===Usage and Biology===
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Can be used as a reporter. Production of RFP in E. coli under the control of a T7 promoter (part subcloned into pUC19) shown here as "RFP2":
  
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https://static.igem.org/mediawiki/2014/b/bc/UV_light.jpg
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https://static.igem.org/mediawiki/2014/d/d7/20141015_174513.jpg
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The excitation and emission spectra match the specifications on the DNA2.0 website. See 'experience' tab above for spectra.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1429001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1429001 SequenceAndFeatures</partinfo>

Latest revision as of 00:34, 22 October 2014

Red Fluorescent Protein (RFP) "Rudolph" coding region, intellectual property-free

IP free synthetic non-Aequorea red fluorescent protein with ribosome binding site.

Usage and Biology

Can be used as a reporter. Production of RFP in E. coli under the control of a T7 promoter (part subcloned into pUC19) shown here as "RFP2":

UV_light.jpg

20141015_174513.jpg

The excitation and emission spectra match the specifications on the DNA2.0 website. See 'experience' tab above for spectra. Sequence and Features Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 147
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2