Difference between revisions of "Part:BBa K1429003"
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Can be used as a reporter. Production of OFP in E. coli under the control of a T7 promoter (part subcloned into pUC19) shown here: | Can be used as a reporter. Production of OFP in E. coli under the control of a T7 promoter (part subcloned into pUC19) shown here: | ||
| + | https://static.igem.org/mediawiki/2014/b/bc/UV_light.jpg | ||
| + | https://static.igem.org/mediawiki/2014/d/d7/20141015_174513.jpg | ||
The excitation and emission spectra match the specifications on the DNA2.0 website. See 'experience' tab above for spectra. | The excitation and emission spectra match the specifications on the DNA2.0 website. See 'experience' tab above for spectra. | ||
Revision as of 00:23, 22 October 2014
Orange Fluorescent Protein (OFP) "Yukon" coding region with RBS, intellectual property-free
IP free synthetic non-Aequorea orange fluorescent protein with ribosome binding site.
Usage and Biology
Can be used as a reporter. Production of OFP in E. coli under the control of a T7 promoter (part subcloned into pUC19) shown here:
The excitation and emission spectra match the specifications on the DNA2.0 website. See 'experience' tab above for spectra.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2