Difference between revisions of "Part:BBa K1431813:Experience"
Zhangysh1995 (Talk | contribs) (→Applications of BBa_K1431813) |
Zhangysh1995 (Talk | contribs) (→Applications of BBa_K1431813) |
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===Applications of BBa_K1431813=== | ===Applications of BBa_K1431813=== | ||
| − | After we constructed this part, we tranformed into BL21 & DH5α to test expression effeciency. In the meantime, we did a comparison between BBa_K1431813 and another part with promoter BBa_J23106, a | + | After we constructed this part, we tranformed into BL21 & DH5α to test expression effeciency. In the meantime, we did a comparison between BBa_K1431813 and another part with promoter BBa_J23106, a relatively weak promoter. |
==LB agar plate growth comparison== | ==LB agar plate growth comparison== | ||
Revision as of 00:06, 22 October 2014
Contents
Applications of BBa_K1431813
After we constructed this part, we tranformed into BL21 & DH5α to test expression effeciency. In the meantime, we did a comparison between BBa_K1431813 and another part with promoter BBa_J23106, a relatively weak promoter.
LB agar plate growth comparison
We spread the plate for BL21 at 10:00am on 15th, Oct and DH5α at 11:00am on 15th, Oct. The plate contains 34ug/ml chloramphenicol. Following pictures were taken every several hours after placing the plates into a 37℃ incubator.
JA is J23100,JB is J23106.
At 00:30 (16th,Oct.)
At 1:50 (16th,Oct.)
At 3:00 (16th,Oct.)
At 6:00 (16th,Oct.)
At 7:40 (16th,Oct.)
At 8:00 (16th,Oct.)
At 10:00 (16th,Oct.)
At 11:20 (16th,Oct.)
LB broth growth comparison
We isolated single colonies from the plates and incubate in 2ml LB broth with 34ug/ml chloramphenicol. Then we isolated single colonies from the plates.
At 00:50 (17th,Oct.)
At 02:45 (17th,Oct.)
At 04:55 (17th,Oct.)
At 22:40 (17th,Oct.)
Conclusion
Unfortunately, BBa_K1431813 didn't grow and there isn't any color on plates or in broth!
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