Difference between revisions of "Part:BBa K1321359:Design"
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===Source=== | ===Source=== | ||
| − | + | The LacI part came from BBa_J04500. | |
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| + | Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part. | ||
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| + | dCBD with its linker were synthesized from Geneart and cloned into the psB1C3 backbone. | ||
===References=== | ===References=== | ||
Revision as of 23:37, 21 October 2014
sfGFP fused to dCBD driven by LacI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 230
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 245
Design Notes
sadf
Source
The LacI part came from BBa_J04500.
Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part.
dCBD with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.