Difference between revisions of "Part:BBa K1321356:Design"
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===Source=== | ===Source=== | ||
| − | + | The LacI part came from BBa_J04500. | |
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| + | Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part. | ||
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| + | CBDclos was requested from the registry, as it was submitted by the Freiburg team as BBa_K863111. | ||
===References=== | ===References=== | ||
Latest revision as of 23:34, 21 October 2014
sfGFP fused to CBDclos driven by LacI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 230
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 245
Design Notes
Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker. The LacI vector was fused to the protein fusion using the Xba and SpeI site.
Source
The LacI part came from BBa_J04500.
Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part.
CBDclos was requested from the registry, as it was submitted by the Freiburg team as BBa_K863111.