Difference between revisions of "Part:BBa K1321354:Design"

 
(Source)
Line 13: Line 13:
 
===Source===
 
===Source===
  
asdf
+
The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part.
 +
 
 +
Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part.
 +
 
 +
CBDclos was requested from the registry, as it was submitted by the Freiburg team as BBa_K863111.
  
 
===References===
 
===References===

Revision as of 23:31, 21 October 2014


sfGFP fused to CBDclos driven by T7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 53
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 68


Design Notes

asdf


Source

The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part.

Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part.

CBDclos was requested from the registry, as it was submitted by the Freiburg team as BBa_K863111.

References