Difference between revisions of "Part:BBa K1321350:Design"

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===Source===
 
===Source===
  
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CBDcenA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone.
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Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part.
  
 
===References===
 
===References===

Latest revision as of 23:30, 21 October 2014


CBDcenA with linker fused to sfGFP in Freiburg format (RFC 25)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 382


Design Notes

Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker.

Source

CBDcenA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone. Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part.

References