Difference between revisions of "Part:BBa K1321154:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Codon optimised for E.coli. | |
| − | + | Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker. | |
| − | + | ||
===Source=== | ===Source=== | ||
Latest revision as of 22:08, 21 October 2014
CBDclos fused to metallothionein Fmt
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker.
Source
Our fMT was a modification of BBa_K190019, wherein we transformed it into Freiburg format. CBDclos was requested from the registry, as it was submitted by the Freiburg team as BBa_K863111.