Difference between revisions of "Part:BBa K1321152:Design"

(Design Notes)
(Source)
 
Line 11: Line 11:
  
 
===Source===
 
===Source===
 
+
Our fMT was a modification of BBa_K190019, wherein we transformed it into Freiburg format.
GATC
+
CBDcex was requested from the registry, as it was submitted by the Freiburg team as BBa_K863101.
  
 
===References===
 
===References===

Latest revision as of 22:04, 21 October 2014


metallothionein Fmt fused to CBDcex


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker.

Source

Our fMT was a modification of BBa_K190019, wherein we transformed it into Freiburg format. CBDcex was requested from the registry, as it was submitted by the Freiburg team as BBa_K863101.

References