Difference between revisions of "Part:BBa K1321150:Design"
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===Source=== | ===Source=== | ||
| − | + | Our fMT was a modification of BBa_K190019, wherein we transformed it into Freiburg format. | |
===References=== | ===References=== | ||
Revision as of 22:00, 21 October 2014
Metallothionein Fmt fused to linker-dCBD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 204
Design Notes
Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker.
Source
Our fMT was a modification of BBa_K190019, wherein we transformed it into Freiburg format.