Difference between revisions of "Part:BBa K1321138:Design"
(→Design Notes) |
(→Source) |
||
| Line 10: | Line 10: | ||
===Source=== | ===Source=== | ||
| − | + | Our SmtA was a modification of BBa_K19010, wherein we transformed it into Freiburg format. The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part. | |
| − | + | ||
===References=== | ===References=== | ||
Latest revision as of 21:56, 21 October 2014
Metallothionein SmtA with T7 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 53
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The T7 vector was fused to SmtA using the Xba and SpeI site.
Source
Our SmtA was a modification of BBa_K19010, wherein we transformed it into Freiburg format. The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part.