Difference between revisions of "Part:BBa K1321122"

Revision as of 21:37, 21 October 2014

Smt metallothionein fused to CBDclos

Metallothionein (metal binding peptide) SmtA fused N-terminally to CBDcex (a cellulose-binding domain).

This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below:

Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
-<partinfo>BBa_K1321122 short</partinfo>
+<partinfo>BBa_K1321121 short</partinfo>
+Metallothionein (metal binding peptide) SmtA fused N-terminally to CBDcex (a cellulose-binding domain).
+This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: [[File:IC14-SmtA-part-table.PNG]]
-GATC
+Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.
 <!-- Add more about the biology of this part here
@@ Line 10: / Line 14: @@
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K1321122 SequenceAndFeatures</partinfo>
+<partinfo>BBa_K1321121 SequenceAndFeatures</partinfo>
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
-<partinfo>BBa_K1321122 parameters</partinfo>
+<partinfo>BBa_K1321121 parameters</partinfo>
 <!-- -->