Difference between revisions of "Part:BBa K1321107:Design"

 
(Source)
Line 13: Line 13:
 
===Source===
 
===Source===
  
CBD15+PhytoC
+
Phytochelatin and CBDcenA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone. The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part.
  
 
===References===
 
===References===

Revision as of 20:00, 21 October 2014


CBDcen-linker fused to Phytochelatin (PC) EC20 with T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 53
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CBD15+PhytoC


Source

Phytochelatin and CBDcenA with its linker were synthesized from Geneart and cloned into the psB1C3 backbone. The T7 vector was made by inverse PCR of BBa_I746909, and ligated. Please see our BBa_ K1321338 for more information on this part.

References