Difference between revisions of "Part:BBa K1321095:Design"

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===Design Notes===
 
===Design Notes===
 
Codon optimised for E.coli.  
 
Codon optimised for E.coli.  
−
Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and SpeI site, creating a scar that also functions as a small linker.
+
Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker.
  
 
===Source===
 
===Source===

Latest revision as of 19:41, 21 October 2014


CBDcex fused to Phytochelatin (PC) EC20


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 333


Design Notes

Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker.

Source

Phytochelatin was synthesized from Geneart and cloned into the psB1C3 backbone. CBDcex was requested from the registry, as it was submitted by the Freiburg team as BBa_K863101.

References