Difference between revisions of "Part:BBa K1321092:Design"
(→Source) |
|||
| Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
| − | Phytochelatin was synthesized from Geneart and cloned into the psB1C3 backbone. CBDcex was requested from the registry, as it was submitted by the Freiburg team as | + | Phytochelatin was synthesized from Geneart and cloned into the psB1C3 backbone. CBDcex was requested from the registry, as it was submitted by the Freiburg team as BBa_K863101. |
===References=== | ===References=== | ||
Revision as of 18:12, 21 October 2014
Phytochelatin (PC) EC20 fused to CBDcex
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and SpeI site, creating a scar that also functions as a small linker.
Source
Phytochelatin was synthesized from Geneart and cloned into the psB1C3 backbone. CBDcex was requested from the registry, as it was submitted by the Freiburg team as BBa_K863101.