Difference between revisions of "Part:BBa K1321092:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1321092 short</partinfo> | <partinfo>BBa_K1321092 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Codon optimised for E.coli. | |
| + | Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and SpeI site, creating a scar that also functions as a small linker. | ||
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===Source=== | ===Source=== | ||
| − | + | Phytochelatin was synthesized from Geneart and cloned into the psB1C3 backbone. CBDcex was requested from the registry, as it was submitted by the Freiburg team as | |
===References=== | ===References=== | ||
Revision as of 18:10, 21 October 2014
Phytochelatin (PC) EC20 fused to CBDcex
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and SpeI site, creating a scar that also functions as a small linker.
Source
Phytochelatin was synthesized from Geneart and cloned into the psB1C3 backbone. CBDcex was requested from the registry, as it was submitted by the Freiburg team as