Difference between revisions of "Part:BBa K1336007"

Latest revision as of 17:52, 21 October 2014

LacI Expression Cassette with BsDyP gene

BsDyP (BSU38260; accession number, NC_000964.3) gene (1251bp) from Bacillus subtilis, found to have been effective in degrading lignin and azo dyes, in an oxidative process. The range of substrates for this enzyme seems to be rather wide, including azo-dyes, phenolic compounds and other aromatics, as well as manganese and ferrous ions. The range of substrates and the mode of action of this enzyme suggests that it could be classified as a bacterial lignin peroxidase. This gene is ligated to a LacI expression cassette, IPTG inducible promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 126
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1741
Illegal BsaI site found at 1750
Illegal BsaI site found at 2716
Illegal SapI site found at 2146
Illegal SapI site found at 2504
Illegal SapI.rc site found at 2660

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 __NOTOC__
 <partinfo>BBa_K1336007 short</partinfo>
-BsDyP gene (1251bp) from Bacillus subtilis that has been found to be effective in degrading lignin and azo dyes, in an oxidative process. This gene is ligated to a LacI expression cassette, IPTG inducible promoter.
+BsDyP (BSU38260; accession number, NC_000964.3) gene (1251bp) from Bacillus subtilis, found to have been effective in degrading lignin and azo dyes, in an oxidative process. The range of substrates for this enzyme seems to be rather wide, including azo-dyes, phenolic compounds and other aromatics, as well as manganese and ferrous ions. The range of substrates and the mode of action of this enzyme suggests that it could be classified as a bacterial lignin peroxidase.  This gene is ligated to a LacI expression cassette, IPTG inducible promoter.
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